Jon Andersen’s free peptide calculator does two jobs. Use the reconstitution calculator to turn any vial into an exact syringe dose — enter your peptide amount, the bacteriostatic water you added, and your target dose to get the precise units to draw, the concentration, and how many doses each vial holds. Or switch to Find My Protocol for the dose and full peptide stack Jon actually runs for your goal.
How this peptide dosage calculator works
The calculator converts your vial into an exact, repeatable dose. It takes three inputs — the amount of peptide in the vial (in mg or mcg), the volume of bacteriostatic water you reconstituted it with, and your target dose — and returns the number of units to draw on a U-100 insulin syringe, the injection volume in mL, the concentration, and how many doses the vial holds. It is a real reconstitution tool, not a generic chart.
How to reconstitute a peptide (step by step)
- Draw the bacteriostatic water into a syringe (the mL you plan to add).
- Inject the water slowly down the inside wall of the peptide vial — aim it at the glass, not directly onto the powder.
- Swirl gently until fully dissolved. Never shake, as agitation can damage the peptide.
- Enter the vial amount, water volume, and your target dose into the calculator above.
- Draw the number of units it shows on your U-100 insulin syringe.
- Store the reconstituted vial refrigerated and use within its stability window.
Peptide dosage reference chart
Common starting doses for popular research peptides. These are general references — always confirm with the calculator and start at the lowest effective dose.
| Peptide | Typical dose | Frequency | Common vial |
|---|---|---|---|
| BPC-157 | 250–500 mcg | 1–2× daily | 5 mg |
| TB-500 | 2–2.5 mg | 2× per week | 5 mg |
| BPC-157 / TB-500 blend | 1 mg | daily (30 days) | 10 mg |
| IGF-1 LR3 | 20–100 mcg | training days | 1 mg |
| CJC-1295 + Ipamorelin | 100 mcg each | nightly | 5 mg |
| Semaglutide | 0.25–1 mg | weekly | 5 mg |
| Tirzepatide | 2.5–10 mg | weekly | 10 mg |
| AOD-9604 | 300 mcg | daily, AM fasted | 5 mg |
| MOTS-c | 1.5 mg | 2–3× per week | 10 mg |
| SS-31 | 1 mg | daily | 10 mg |
| Semax | 500 mcg | 2× daily | 5 mg |
| Epitalon | 2–3 mg | nightly (cycles) | 50 mg |
| GHK-Cu | 1–2 mg | daily | 50 mg |
| PT-141 | 1–2 mg | as needed | 10 mg |
Unlock your full peptide stack
The reconstitution calculator is free and ungated. When you use the Find My Protocol tab, the calculator shows your goal’s primary compound and starting dose free, then unlocks the complete stack Jon actually runs — the synergistic add-ons, dosing for each, and direct links to lab-tested vials — after you enter your email.
Frequently asked questions
How do you calculate peptide dosage?
Divide the amount of peptide in the vial by the bacteriostatic water you added to get the concentration, then divide your target dose by that concentration to get the volume to inject. On a U-100 insulin syringe, 100 units equals 1 mL, so multiply the volume in mL by 100 to get the units to draw. The calculator above does all of this instantly.
How much bacteriostatic water should I add to a peptide vial?
Most people add 1–3 mL of bacteriostatic water to a standard 5 mg or 10 mg vial. More water makes the dose easier to measure precisely; less water means a smaller injection volume. The exact amount is your choice — just enter it into the calculator and it recalculates the units to draw.
How many units are on an insulin syringe?
A standard U-100 insulin syringe holds 100 units per 1 mL. A 0.5 mL barrel shows 50 units and a 0.3 mL barrel shows 30 units. The calculator lets you pick your syringe so the units it shows match your barrel.
How do I reconstitute BPC-157 and other peptides?
Inject bacteriostatic water slowly down the inside wall of the vial onto the powder, swirl gently until dissolved (never shake), then draw your dose. Follow the step-by-step guide below and use the calculator to get the exact units.
Is this peptide calculator free?
Yes. The reconstitution and dosage calculator is completely free with no sign-up. You only enter your email if you want to unlock Jon Andersen’s full recommended stack for a specific goal.
What peptides does Jon Andersen recommend?
Jon’s protocols cover fat loss (AOD-9604), injury and healing (BPC-157/TB-500), muscle (IGF-1 LR3), the growth-hormone axis (CJC-1295/Ipamorelin), energy and longevity (SS-31/MOTS-c), focus (Semax), skin (GHK-Cu), sleep (Epitalon) and libido (PT-141). Use the Find My Protocol tab to see the full stack for your goal.
Get your personalized peptide protocol
Ready to run it the right way? Shop Jon’s lab-tested peptides → — third-party tested and shipped from a verified U.S. source. Or explore Jon’s full protocol library and AI calculator →.